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Objective@#To investigate the expression of long non-coding RNA (lncRNA) SNHG8 in EB virus related gastric cancer and their correlation prognosis.@*Methods@#The expression of SNHG8 in 93 gastric cancers and 93 cancer-free controls, matched by age and sex, were determined by real-time PCR. EB virus expression was detected by EBER in situ hybridization.@*Results@#Forty-one gastric cancers were EB virus associated. For all gastric cancers, SNHG8 expression was 14 times higher (P=0.001) than that in non-cancer controls; in the EB virus related gastric cancers, SNHG8 expression was increased 25 times (P<0.05) over EB virus negative gastric cancers. SNHG8 expression level was also significantly associated with TNM staging (P<0.05).@*Conclusions@#SNHG8 may act as a proto-oncogene, participating in gastric carcinogenesis.EB virus infection of gastric mucosa may promote SNHG8 expression.
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Objective To investigate the efficacy and safety of dezocine applicated in postoperative analgesia for elderly patients.Methods 50 elderly patients with elective cholecystectomy were selected as the research object,and they were randomly divided into the study group and control group.The control group patients took sufentanil + tropisetron treatment,while the study group took sufentanil + dezocine + tropisetron treatment.The postoperative analgesia sedation score,adverse reaction of the two groups were observed and recorded,and the contrast analysis was carried on.Results There was no significant difference in comparison of postoperative analgesia sedation score of the two groups (P ＞ 0.05) ; The incidence of adverse reaction of the study group was 12％,which was significantly lower than 40％ of the control group(x2 =20.37,P ＜ 0.01).Conclusion Dezocine for postoperative elderly patients has obvious effect,postoperative analgesia and sedation,and high safety,which is worthy of reference.
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Objective To explore the differential expression of microRNAs (miRNAs) in Burkitt lymphoma (BL) and their potential use as biomarkers for BL diagnosis.Methods The different miRNAs in BL from reactive hyperplasia of lymph node cases were screened by miRNA chip.The potential targets of miRNAs were predicted using miRWALK.MAS3 program was used to determine the putative functions of potential miRNA target genes by annotation using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses.Results A total of 46 miRNAs (36 upregulated and 21 downregulated) were dysregulated in BL compared with reactive hyperplasia of lymph node.Interestingly,members of the let-7 family (let-7f-1-3p) was downregulated in BL.The target genes of let-7f-1-3p were predicted,and the GO enrichment analysis revealed their functions were mainly related with multicellular organismal development and regulation of transcription from RNA polymerase Ⅱ promote.KEGG pathway analysis was also carried out among the predicted target genes,which showed that they were mainly involved in TGF-beta signaling pathway and related with chronic myeloid leukemia.Conclusions The set of differentially expressed miRNAs identified here expands the range of potential diagnostic markers for BL.
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<p><b>OBJECTIVE</b>To investigate the methylation status of Runx3 promoter and Runx3 expression in breast lesion tissues.</p><p><b>METHODS</b>One hundred and fourteen breast lesions, including 35 cases of fibroadenoma, 39 cases of intraductal carcinoma, 40 cases of invasive ductal carcinoma, and 33 cases of normal breast tissue from Fabruary 2010 to August 2012 were included in this study. Runx3 protein expression was assessed by immunohistochemical SP method; whereas methylation of Runx3 promoter was assessed by high resolution melting (HRM) analysis.</p><p><b>RESULTS</b>Runx3 protein was mainly expressed in the cytoplasm of ductal epithelial cells. The expression rates of Runx3 in normal breast tissue, fibroadenoma, ductal carcinoma in situ, invasive ductal carcinoma were 87.9% (29/33), 85.7% (30/35), 53.8% (21/39), and 40.0% (16/40) respectively. The methylation rates of Runx3 promoter were 12.1% (4/33), 20.0% (7/35), 46.2% (18/39), and 57.5% (23/40), respectively. Correlation analysis between promoter methylation and protein expression of Runx3 in different breast tissue showed the r value in normal breast tissue, fibroadenoma, ductal carcinoma in situ and invasive ductal carcinoma was -0.431 (P = 0.012), -0.408 (P = 0.015), -0.589 (P = 0.000) and -0.743 (P = 0.000) respectively.</p><p><b>CONCLUSIONS</b>Runx3 protein expression shows a downward trend in ductal carcinoma in situ and invasive ductal carcinoma, meanwhile its promoter methylation increases significantly. The methylation of Runx3 promoter may be one of the important factors in the occurrence and development of breast cancer.</p>
Subject(s)
Female , Humans , Breast , Metabolism , Breast Neoplasms , Metabolism , Carcinoma, Ductal, Breast , Metabolism , Carcinoma, Intraductal, Noninfiltrating , Metabolism , Core Binding Factor Alpha 3 Subunit , Genetics , Metabolism , DNA Methylation , Fibroadenoma , Metabolism , Neoplasm Proteins , Metabolism , Promoter Regions, GeneticABSTRACT
OBJECTIVE:To study the pharmacokinetics of Cefoperazone in dogs and compare the pharmacokinetic differ?ence between single Cefoperazone and Cefoperazone plus Tazobactam.METHODS:Six healthy dogs were given intravenously a single dose of Cefoperazone Sodium and Cefoperazone-Tazobactam Sodium.Levels of Cefoperazone in dogs'serum were de?termined by RP-HPLC method.The pharmacokinetic parameters were obtained and compared between Cefoperazone Sodium and Cefoperazone-Tazobactam Sodium.RESULTS:The serum drug concentration-time course in dogs was conformed to the two-compartment model after a single dose of Cefoperazone Sodium and Cefoperazone-Tazobactam Sodium.Area under the concentration-time curve(AUC 0～∞ )was(486.17?117.98)、(469.74?71.49)(mg?h)/L,half-time(T 1/2? )was(0.96? 0.13)、(0.85?0.17)h,respectively.There were no significant differences between two groups.CONCLUSION:Taking AUC 0～∞ as index,two preparations are bioequivalent.